FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937635
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG5650.021.375814
GTCCTAC3850.016.1329861
GTATTAG12150.015.6203011
TAGGTAG6150.015.1500415
TCCGCAC4650.014.83854464
GCAGCGT8150.014.8188121
CAGCGTC8550.014.1260172
CTATCCC7200.013.4192584
GCGCTCG3801.902663E-812.71016933
CCCCGGC2801.9658006E-512.32108223
CCGCACC5054.7293724E-1112.29689365
GTATATA34300.012.2735081
GTATAGA13500.012.2690361
CTAATAC13600.012.17882253
CCCTCGG3154.9445753E-612.048518
CCTTGCG9450.012.0478959
CCTATAC10650.011.9882323
GCTCGCG5501.6370905E-1111.91784712
TAATACT24350.011.9037374
ACTGTGC7000.011.8294478
Produced by FastQC (version 0.11.2)