FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201574
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG9000.019.554154
GTATTAG18600.016.3256551
GCAGCGT11700.015.6311491
CCGCACC7950.014.75433565
TCCGCAC7650.014.43099964
CGCACCT7950.014.32038566
CAGCGTC12700.014.1288142
TAGGTAG10100.012.6414475
GTATATA45200.012.4437151
AGCGTCA14750.012.3992413
TATACAG26200.012.3806375
ACTATGC19000.011.803178
GACCGAG14600.011.57846769
GGCGCGG2100.00446579811.49970247
CCTATAC13900.011.4196893
CGTCAGA16100.011.3596885
TAATACT35700.011.3091214
CCCGGGG2450.001096000811.265827
CCCCAGG4351.2424061E-711.1055391
GTATAAA32150.010.8402991
Produced by FastQC (version 0.11.2)