FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3769404
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG8550.025.4246444
CAGCGTC10450.021.4623642
GCAGCGT10500.021.0321031
AGCGTCA12600.017.2524383
GTATTAG16200.015.9749031
CGCACCT6200.014.467483566
TAGGTAG9600.014.0177925
GATGTGT16400.013.88408310
CTAATAC19700.013.4866783
CGTCAGA16300.013.3364175
TAATACT30700.013.0376724
GTATATA42650.013.0256321
CTGTGCG4053.3778633E-912.7777199
CCGCACC6800.012.68359765
TCCGCAC6450.012.30210664
TCGGGGG3154.9506925E-612.04740416
GAGACAG17600.011.95717221
CGGTCGG2902.8166744E-511.89633913
TAGTCAG8150.011.8527069
TAGACAG12100.011.691895
Produced by FastQC (version 0.11.2)