FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587894
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT9400.020.1898781
GCGTCAG9700.019.2097114
CAGCGTC10250.018.515082
GTATTAG14600.016.7804781
TAGGTAG7800.014.1566435
AGCGTCA13500.013.8025343
TATACGG13600.013.7012365
CCGTGCA5600.013.5541719
GTATAGA16350.012.8739221
TAGTCAG7650.012.628019
CGTCAGA15900.012.1533445
GAGACAG16300.012.06411121
ACGGTGT11450.012.0529358
TACGGTG12600.012.0481517
GGTAGTC8900.012.01738557
CCCTTAG9800.011.6194811
GTATTAT30400.011.4642751
ATACGGT14150.011.4616296
GATGTGT17300.011.16765710
CGCGCAT4351.2458622E-711.10316640
Produced by FastQC (version 0.11.2)