FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077959
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG6450.024.6071364
CGCACCT6750.019.9330866
TAGGTAG8550.019.7742485
GCAGCGT8200.018.9351461
TCCGCAC7300.018.9038764
CAGCGTC8300.018.7067092
GTATTAG15300.018.4923651
CCGCACC7100.018.46455465
TAGTCAG7650.018.039289
GGTAGTC9050.016.7740867
ACATAGG9600.015.09355831
ACTATGC15300.014.882898
AGCGTCA11050.014.3634423
ATAGGAG9850.014.36022433
CTAATAC16650.014.0915153
CGTCAGA11800.013.450735
ATCAGCC10600.013.3441754
TAATACT27150.013.3438894
GATGTGT12450.013.30125210
CCTAATA18500.013.2418782
Produced by FastQC (version 0.11.2)