FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432099
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC4600.017.99962464
CGCACCT5150.015.40744666
TATACCG7050.015.1730175
CCGCACC5600.014.16934865
CTAATAC12000.014.0895233
CGCGCAC1750.001135308313.80027910
CGAGACG12150.013.62934669
GTATAGA11650.013.6239941
TATACGG8400.013.1452865
GTATTAG10250.013.1284461
ACCGCGC1850.001725820813.0545868
CCTAATA12950.012.7892192
TAGGTAG6750.012.7801395
GTCCTAA5651.8189894E-1212.2138921
TAATACT21050.012.1300084
TCACACA8050.011.9997530
GTATTAT24450.011.8542191
ACTATGC11900.011.5971128
TATATAC32000.011.5375943
CCATGCG3601.7374023E-611.5004699
Produced by FastQC (version 0.11.2)