FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866774
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG5650.017.7117394
CGCACCT5900.016.957166
CCGCACC6500.016.45333565
TCCGCAC7050.014.68039564
CAGCGTC7150.014.4786112
CCGCGCG2657.103772E-714.3208099
CTAGGAC4101.8189894E-1114.3076773
TATACTG20550.013.7693585
GCAGCGT7650.013.0803051
ACGGTGT10300.013.0645248
AGCGGGG3357.294475E-712.3580450
TAATACT24650.012.3190564
TACGGTG10700.012.2536667
GTATTAG14500.012.1362221
AGCGTCA8550.012.1076323
ACCGCGC2000.003097430812.076318
TATACGG12350.012.0147075
TATAATA47050.011.73472
CTATGCA12350.011.7328399
TTATACT20950.011.5299034
Produced by FastQC (version 0.11.2)