FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089915
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG8000.019.412164
GCAGCGT8250.018.8229981
CGCACCT5950.017.39462366
CAGCGTC9900.016.0349242
CCGCACC6350.015.75560265
TAGGTAG7600.015.439165
GTATTAG14350.014.9097381
TCCGCAC6800.014.71295364
AGCGTCA11600.013.68523
CGTCAGA11400.013.6227885
GGTAGTC8050.013.2863197
CTATACT14050.013.2638254
TATACTG19300.013.2322215
CTAATAC16450.013.0069993
TAATACT26150.012.9331914
TAGTCAG7300.012.7602369
TAGGACA8300.012.4736774
GATGTGT14400.011.97893710
GTATTAC14450.011.9407721
ACTATGC15050.011.6913728
Produced by FastQC (version 0.11.2)