FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227894
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9100.016.3051091
GCGTCAG5300.015.6257834
CCTAGGA2705.2417818E-815.3364162
TCTATAC10850.014.3116323
TATACTG14950.014.080075
GCAGCGT5900.013.4515541
CAGCGTC6200.013.3575242
TAGGTAG5450.013.2965495
GTATATA24600.013.0450121
TGACGCG2701.3525259E-512.77747837
CTAGGAC3255.037891E-712.7410223
CGTCTCG1900.00210964212.71051410
TATACAG14600.012.5267725
GACGCGC2509.3389426E-512.41970938
GTATATG15050.012.3809481
CTGAGCG2306.45321E-411.9999889
TATTAGG10000.011.7323572
TAGTCAG5005.16593E-1011.7299889
CCCTTAG6007.2759576E-1211.5020541
CCGCGGA2100.00446384411.49998959
Produced by FastQC (version 0.11.2)