FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114734
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13550.019.0981241
GCGTCAG6350.019.0197534
TCCGCAC5550.018.64808564
TAGGTAG8300.017.8778325
CGCACCT5900.017.54212466
CCGCACC6200.017.24947765
GCAGCGT7500.015.6417871
TAGTCAG7750.015.5814259
GGTAGTC9450.014.9699647
CAGCGTC8600.014.0429792
GTCCTAC4001.9099389E-1013.8015771
ATAGGAG9400.013.21257933
TTAGGTA14850.012.5480974
CTTACAC7500.012.4224163
TAGACAG9200.012.3780035
TATTAGG16250.012.3158472
ACATAGG9350.012.17629831
ATTATAC23800.012.0338533
TCTATAC16100.012.0023353
TCCTATA14450.011.9396552
Produced by FastQC (version 0.11.2)