FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330122
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG7250.017.1332684
TCCGCAC5700.014.52602864
CAGCGTC9250.013.8015922
GTATATA42850.013.5278141
GCAGCGT9450.013.5094941
CGCACCT6450.013.37182866
CCGCACC6200.013.35457465
GGGGCTC2751.6335614E-512.54539410
ACGCGGG2204.4407428E-412.54520565
GTATAGA16200.012.3532761
TATACAG22650.012.3393565
GCTCGCG5054.7293724E-1112.2969761
TACTATG17450.012.258747
CTAATAC15650.012.1261813
AGCGTCA10350.012.0015643
GTATTAG13550.011.7135281
GTATATG23100.011.5013271
ATACAGG14500.011.422356
GTCCTAC4551.8950232E-811.3749391
TATATAC48950.011.3487723
Produced by FastQC (version 0.11.2)