FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131296
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG5100.019.6210774
CAGCGTC5500.018.193662
GTATTAG9650.017.5211981
GGACCGC1200.001475145617.2538266
GCAGCGT6000.016.6779141
CCGCACC3209.822543E-1116.17166565
AGCGTCA6450.015.5143393
TATACTG15300.014.6601135
CGCACCT3657.1304385E-1014.17789866
TAGGTAG5600.014.1727855
CCGCCCG2001.9821365E-413.80046810
TCCGCAC3751.0677468E-913.79982264
CACCTAG3253.3825927E-813.79949828
GTCGGGG1550.00811543713.35435315
TACACAG5700.013.3187425
GGTAGTC4951.8189894E-1213.2438067
ACCTAGC3457.395283E-812.99952729
CGCTACG4852.3646862E-1112.80395969
TATTAGG12150.012.7797122
CCTACAC3255.0391463E-712.7406883
Produced by FastQC (version 0.11.2)