FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173273
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12050.019.4726834
CGCACCT10300.018.4221966
CCGCACC11000.017.24986865
CAGCGTC14250.016.9505162
TCCGCAC11050.016.859664
GCAGCGT14450.016.2381531
GTATTAG25600.015.2311971
TAGGTAG13500.015.08116155
CTAATAC25000.014.3546663
TAGTCAG12650.014.1819859
TATACGG20650.013.869945
GGTAGTC12500.013.8005637
TACGGTG16200.013.2042427
AGCGTCA18050.012.9996443
ACTATGC21900.012.7606728
TACTATG28300.012.5570497
TAATACT42650.012.3787374
GCGACGT7050.012.23371344
CTATGCA20450.012.1468429
CTTACAC14500.012.1367373
Produced by FastQC (version 0.11.2)