FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3908829
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG8300.022.4511644
CAGCGTC11300.016.4906772
GTATTAG19000.016.1639861
TAGGTAG8150.015.6665325
GCAGCGT11900.015.6588151
ACCGTGC3202.759225E-814.0161168
CGCACCT5550.013.67545666
AGCGTCA14100.013.2159333
TCCGCAC6250.012.69579564
CGTCAGA14950.012.4646855
GGTAGTC9200.012.3754357
TATTAGG20050.011.8756662
CCGCACC6700.011.84309265
ACGCGCG3551.4700672E-611.66163438
GTATAGA18350.011.6591731
GGCGTCG5401.5097612E-1011.49966732
GTCCTAG4853.985406E-911.3838811
TACACCG8800.011.37218955
TAGTCAG10150.011.2168559
GATGTGT17750.011.07883610
Produced by FastQC (version 0.11.2)