FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4887855
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11000.020.0780264
TCCGCAC8150.016.08552764
GCAGCGT13750.015.81063651
TAGGTAG12300.015.1504945
GTATTAG21750.014.9135181
CAGCGTC15150.014.57840252
TAGTCAG11350.014.2861689
TACACCG8400.013.5572885
CGCACCT9700.013.51515966
AGCGTCA17050.012.9534323
GGTAGTC13100.012.9057427
TATTAGG22300.012.84446052
CCGCACC10300.012.39292465
CTAATAC24250.012.0958533
TATACCG14600.012.0546755
GTATTAT42500.011.9354811
TATACTG32150.011.8072815
CTAGGGT5602.3646862E-1111.70844654
ACTATGC18700.011.6240418
GTATATG33050.011.5894561
Produced by FastQC (version 0.11.2)