FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586114
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25100.019.6601891
GCGTCAG12550.017.8725784
TAGGTAG12950.017.3206845
TAGTCAG14650.015.30707559
ACATAGG14200.014.57711731
GCAGCGT15350.013.9382631
TATTAGG26300.013.6455582
CAGCGTC16050.013.5450062
GGTAGTC16350.013.5047487
TATACTG32150.012.4508355
CATAGGA17850.012.36945232
TCCTTGC31350.012.3255058
TTAGGTA21500.012.1981144
CCTTGCG14750.012.162649
TCCGCAC10200.011.83805864
GTATTAT48450.011.8233261
ATAGGAG17550.011.79459133
ATTAGGT24600.011.6428843
TATACAG36200.011.439165
CCGCACC10600.011.3913465
Produced by FastQC (version 0.11.2)