FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583191
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15450.015.6327751
GCGTCAG8250.014.6385764
GCAGCGT8300.014.5497811
GTATAGA14500.014.2774221
CTAGGGT4304.1836756E-1113.6415964
CAGCGTC9050.013.3443712
GTATCCT15200.012.4849151
GTATATA34900.012.1603541
GTCCTAG5156.548362E-1112.059571
GTCCTAC6050.011.9765151
TATACAG19150.011.8927995
TAGGTAG7550.011.8832535
GTATTAT30300.011.8428771
CGGTCAG3802.6050657E-711.80227315
CGGACGG3853.085297E-711.649322549
GTATATG21800.011.5540141
GGACCGT4259.001269E-811.3670576
GTCCTAA7950.011.2842511
CCCTAAG6151.0913936E-1111.2207381
TATTATA40700.011.1907232
Produced by FastQC (version 0.11.2)