FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978036
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG14800.018.4206894
GCAGCGT16350.016.251161
CGCACCT11150.016.08947266
GTATTAG23300.015.5505141
CGCGCGG4350.015.06838654
CAGCGTC17750.014.9701312
TCCGCAC12650.014.18151164
TAGGTAG10750.014.1249955
CCGCACC12950.013.58669165
TCCTTGC39700.012.8623668
GTATCCT28200.012.8484761
CGCGCAT6450.012.83703840
CTATGCA22800.012.7104639
TAATACT44150.012.3499974
AGCGTCA21900.012.2910023
CTAATAC28500.011.9874593
GTATAGA27150.011.947291
CCTAATA28900.011.5826272
GTATTAT47200.011.4780751
CCTATAC18800.011.3807453
Produced by FastQC (version 0.11.2)