FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5476515
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12350.019.5592844
GCAGCGT15000.016.7925931
GTATTAG23600.015.3519461
CAGCGTC17200.014.6455062
TAGGTAG13150.014.4333355
CGCGCGG3801.3042154E-913.61803654
TAGTCAG12950.013.0542829
CCTTGCG16100.012.85736759
AGCGTCA19900.012.4852523
CTAGGGT7800.012.3875464
TCCTTGC35150.012.3681188
GTATAGA25250.012.298931
GGTAGTC14400.011.9802527
CTAATAC27500.011.9208933
CGCACCT9850.011.90829366
GTATCCT23800.011.8883911
CCCCGGC4702.4447218E-911.74445723
GATGTGT23850.011.71697210
CGCGCAT4752.8867362E-911.62072640
ATTATAC39950.011.5745983
Produced by FastQC (version 0.11.2)