FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666123
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG8650.020.7448234
CGCGCGG3651.8189894E-1216.06801654
TAGGTAG10650.014.9051165
GCAGCGT12600.014.7892351
GTATTAG22050.014.5544861
CAGCGTC13350.013.958232
TCCTTGC30550.013.8905268
GACGCGC4851.8189894E-1213.51520538
GTATCCT20150.012.844251
CCTTGCG13150.012.3308279
CGCGCAT5359.094947E-1212.25210340
CGCGGGA4451.36788E-811.6289956
GTATAGA21850.011.5290611
GTATATG29550.011.4443491
ACTAGAC10650.011.3407283
ACGCGCA5906.002665E-1111.10995839
AGCGTCA16900.011.0262943
GTCTTAG13550.010.9509471
CGTCCGG4753.5810444E-810.89452932
CGTCAGA16950.010.7902965
Produced by FastQC (version 0.11.2)