FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5379839
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11550.020.6151894
GTATTAG22400.019.4109481
TAGGTAG13650.017.1911355
GTATAGA24850.015.8307931
GCAGCGT16850.014.5406111
TATACTG36750.014.2729765
CAGCGTC17800.013.3765842
GGTAGTC13050.013.2188877
TATACAG31150.012.8507435
GCGCACT7800.012.82692911
TAGTCAG13450.012.8252849
ACCGCGC3852.2833774E-812.5459258
CCTACAC10900.012.346913
TATTAGG24700.012.2942452
GTATATA53150.012.2710791
CCCGGGG2255.3645286E-412.2671287
CGCACCT9550.011.92124966
AGCGTCA20100.011.8460413
CCTAGAC11450.011.7538283
TCCGCAC10450.011.55470764
Produced by FastQC (version 0.11.2)