FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273364
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23400.018.2858281
GCGTCAG10700.016.1248264
TAGGTAG13750.015.56001955
TAGTCAG14000.013.8002349
CCGCACC9550.013.7274665
GGTAGTC15650.013.668747
TAATACT38900.013.4835054
GCAGCGT13400.013.1333091
TCCGCAC9500.013.07340964
CGCACCT10150.012.91598566
TATTAGG25900.012.7902872
CTAATAC23650.012.5480473
ACATAGG14950.012.46115831
GTCCTAG8850.012.0872431
GACGCGC5156.548362E-1112.05838
TTAGGTA24050.012.0523854
GTACTAG15350.011.6897121
CAGCGTC15300.011.5023752
GTATAGA24800.011.4096151
ATAGGAG16400.011.35940933
Produced by FastQC (version 0.11.2)