FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5607828
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25800.017.3869651
TAGGTAG12950.017.054445
GCGTCAG14000.016.5145344
CCTTGCG18100.015.6299659
CCTCCTA10600.013.9978941
TATACTG34950.013.62571055
TATTAGG24700.013.4118872
CTACCCC10550.013.4106834
CAGCGTC17500.013.4086952
GTATAGA26150.013.0636241
CGCGCGG3457.402741E-812.99964354
GCAGCGT18550.012.8352791
TTAGGTA19450.012.5967434
GTATATG36900.012.3437671
TATACAG32150.012.3436645
TCTATAC25350.012.2513533
CTATACT28950.012.0390534
ACATAGG13950.011.87064331
TAATACT44300.011.8402094
TCCTTGC36150.011.8348628
Produced by FastQC (version 0.11.2)