FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643097
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24500.016.338521
CGCACCT10600.015.622148566
GCGTCAG13450.014.6250214
TAGGTAG12950.014.3903645
GACGCGC6100.014.13889738
TCCGCAC13100.013.69422364
GCAGCGT15300.013.3070391
CTAATAC27550.013.0273383
GTATATG32550.012.827891
ACCCGCG6350.012.49566833
CCGCACC14650.012.24534765
GTCCTAA14450.012.1793231
TATTAGG23550.012.1627312
GTATAGA24750.012.1301141
CGCGCAT7400.012.12123940
TAGTCAG12750.011.9057179
TAATACT40650.011.8853354
TAGACAG18850.011.7169995
GGTAGTC14050.011.5416857
CTAGACT9300.011.5033054
Produced by FastQC (version 0.11.2)