FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4531223
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG9850.020.3208565
GTATTAG19150.017.480281
GCGTCAG10800.016.2960474
GTATCCT20650.016.0434071
TATTAGG17750.014.5812242
CGCGCGG3657.1304385E-1014.17751254
TTAGGTA15750.014.0228184
GCAGCGT12950.013.8573131
CCTCCTA9400.013.216611
CAGCGTC13950.012.8635322
TCCTTGC29950.012.6718688
TAGTCAG10700.012.5750959
CCCCTAG4701.8371793E-1012.4823541
GTATAGA19600.012.3250141
TATACTG26350.012.3111175
CTTCCTA19450.012.2426361
GTCCTAG7650.012.1800141
GGTAGTC11400.012.1059797
TCCTATA19200.012.0421672
CTATACT21000.011.8317534
Produced by FastQC (version 0.11.2)