FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335037
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG15450.018.7617474
TAGGTAG15750.018.1855685
TCCGCAC13350.016.53895664
GTATTAG27950.016.4211141
CGCACCT12600.016.154466
CCGCACC13650.015.41723565
GCAGCGT19800.014.8144831
TAGTCAG14650.014.3650939
CAGCGTC20500.014.3081712
GGTAGTC16700.013.6349637
CTAATAC29950.013.4806723
TATTAGG27200.013.4479482
TTAGGTA26450.013.3075154
ATTAGGT28600.013.0310743
ACATAGG16000.012.7215931
TAATACT48450.012.677944
GTATAGA31650.012.647861
TATACAG37550.012.4066115
GTCCTAA15250.011.9933031
ATAGGAG18250.011.90932233
Produced by FastQC (version 0.11.2)