FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6229610
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12550.018.9720344
GTATTAG26950.016.772591
TAGGTAG15000.015.6433465
CGCGCGG4650.015.58022654
CAGCGTC17000.014.4114432
CGCACCT12850.014.22941966
TGTTAGG16550.014.1778042
GCAGCGT17650.013.4893811
CCTCCTA11300.013.4357471
TCCTTGC42250.012.9026718
CAGCGCA13400.012.8732019
GGTAGTC16400.012.622937
CTAATAC31450.012.5079393
TCCGCAC13800.012.49986564
CGCGGGA5800.012.49104456
CCGCACC14750.012.1625865
CCTTGCG19050.012.1339229
TCCTAAG17350.012.1318882
TAATACT47850.011.8990014
GCGCACT9900.011.84826111
Produced by FastQC (version 0.11.2)