FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4709699
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11750.020.5568834
GCAGCGT13100.017.9102821
CAGCGTC13550.017.3165782
GTATTAG19700.016.113371
GACGCGC4850.014.93781438
CGCGCGG3051.46828825E-814.70459254
CGCACCT9450.014.6028566
CCGCACC10000.014.48967965
TAGGTAG11550.014.3406935
AGCGTCA17300.013.7625883
TCCGCAC9950.013.52231364
CCTTGCG13200.013.3298159
AGGTCCG3651.083572E-813.23258336
GTATAGA22850.012.2310471
TCCTTGC28100.012.0331688
GTATATA48900.011.9951281
GTATTAT37900.011.8350181
TGACGCG6450.011.7671837
TTATACT29950.011.7516934
TAGTCAG11200.011.7055959
Produced by FastQC (version 0.11.2)