FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5197253
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG11350.021.5868325
GTATTAG21950.018.7081341
GCGTCAG12050.017.1821734
TCCGCAC10500.016.42820564
TATACAG31950.016.4172025
TATACTG31050.015.7816775
CCGCACC11600.015.16776565
CGCACCT12000.014.94966666
TATTAGG23150.014.60782
CTAATAC24900.014.4126453
TAATACT40400.014.2642514
GTATTAT40600.014.1091261
GCAGCGT15400.013.89277651
CTTACAC12550.013.7478893
ATACAGG23800.013.6292876
TATATAC57400.013.4061073
GACGCGC5450.013.29328238
ACATAGG14000.012.81387631
TAGTCAG14300.012.786929
GGTAGTC17050.012.748627
Produced by FastQC (version 0.11.2)