FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803539
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12550.019.7944181
TAGGTAG7850.018.462025
GCGTCAG9550.016.2595584
TATTAGG13000.015.9256112
CGCGCAT6500.015.39192440
GACGCGC6350.014.66892538
TAGTCAG8850.014.4239399
TTATACT19300.013.945594
ACGCGCA6750.013.79965639
GCAGCGT11900.013.6271431
GTATTAT25200.013.5546591
GTATATA25100.013.4712011
CTAATAC14450.013.3727223
CTTAAGG9950.013.1783233
GTATAGA17300.013.1629061
TGACGCG7450.012.9661237
TTAGGTA12000.012.93989854
CCCTCGG6800.012.6850088
CCTCGGA8750.012.6173269
TATACAG18000.012.4606445
Produced by FastQC (version 0.11.2)