FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765920
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3750.018.403575
CTAATAC5950.017.3983353
TATTAGG7050.016.641532
GTATTAG6850.016.6231941
TAATACT10450.016.1801744
GTATATA11450.015.9720791
TCTATAC7600.015.8912423
CCGAGCG1554.525971E-415.58058132
CTGTGCG2001.1426942E-515.5249379
ACCCGCG3001.178887E-814.94993933
TATACAG7750.014.6931755
CCGCGCT3202.7572241E-814.01556835
CCTCGCG1750.001135301213.7999439
CCCGCCG3506.004484E-913.79955323
CTTAGAC6300.013.6931333
CGACGTA3304.130743E-813.59046845
GACCCGC2052.4398397E-413.4660296
TAGACCG6150.013.4660285
GTATTAT10300.013.400281
TACACCG3901.9135769E-913.2718085
Produced by FastQC (version 0.11.2)