FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5183049
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21900.019.3795931
GCGTCAG12950.016.7869264
TAGGTAG12150.016.7565465
GACGCGC6550.016.32799338
CGCGCAT6950.015.88464740
ACGCGCA6750.015.84420139
GCAGCGT14400.015.3356131
TATTAGG20100.015.1068692
TGACGCG7700.014.3375837
GTATAGA22150.014.3317021
TAGTCAG12200.013.8571469
GTATATA35650.013.1632471
TATACTG29500.012.9840375
CTAATAC21300.012.9601733
GTATTAT38450.012.9225981
GGTAGTC15200.012.4843247
CGCACCT9500.012.34717866
CAGCGTC18250.012.2896162
CCTCCTA10400.011.9440821
ACATAGG13300.011.93214831
Produced by FastQC (version 0.11.2)