FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3645425
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG8750.018.1404194
CCCCTAG4650.015.5832481
GACGCGC4201.8189894E-1214.78534438
GTATTAT23650.014.7360751
GTATCCT15850.014.368331
CCTTCTA16350.014.3510211
TATTATA25500.013.8026842
CCCCCTA3253.3760443E-813.8023041
GTATTAG12900.013.6418121
TCCTTGC22700.013.527028
CAGCGTC11750.013.5090082
CTTACAC9750.013.4487693
GCAGCGT12050.013.1723241
TACTCGG6600.013.068212511
CCCTTAG13400.012.8752841
CCCTCGG6200.012.7989448
ACGCGCA5155.456968E-1212.72783839
CGCGCGG3002.8691666E-612.64968454
GCGACGT8200.012.62163544
GGACTGA8500.012.58203732
Produced by FastQC (version 0.11.2)