FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331823
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8400.018.485551
GCGTCAG5550.018.0307374
TAATACT14900.016.6745724
TCTATAC10500.015.77467253
GTATTAT15800.014.6324441
CGCGCAT3152.2437234E-814.23766640
TATAAGG8600.014.0432862
GCAGCGT7050.013.7046541
TCCGCAC4406.002665E-1113.32943164
CCTATAC8550.013.3185293
CGACGTA5450.013.29317745
TCCTTGC14050.013.2605258
CTATACA9400.013.2154854
CTAATAC9400.013.2154853
TATACAG9950.013.1791555
TCCTATA7900.013.1036822
ACGCGCA3457.395829E-812.99960839
TAGGTAG4801.8189894E-1112.9407185
GCGACGT5600.012.9371144
TATTAGG7750.012.9120572
Produced by FastQC (version 0.11.2)