FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794066
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT3250.020.16906440
ACGCGCA3300.018.81802739
GTATTAG9450.017.161661
GACGCGC3501.8189894E-1216.75700438
GCGTCAG6750.016.3580534
TATTAGG9450.015.700812
TAGGTAG5350.015.4795
TGACGCG4250.015.423437
CTAATAC8900.015.1202873
ACCCGCG3201.6989361E-915.09362533
TATACAG11400.014.5285355
GTATTAT18350.014.1032251
CTATACT11800.014.0360424
TATACTG15700.013.8460645
GCGCACT4801.8189894E-1213.65589311
CTATACA12050.013.45848754
CTTAAGG7200.013.4187163
GTATAGA12250.013.2389941
CCGGCCC3651.0828444E-813.2325313
GGACCGT3508.9065E-812.8162436
Produced by FastQC (version 0.11.2)