FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115501
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA17500.015.774551
GTATTAG15500.015.1384811
GCGTCAG10600.014.6489474
GACGCGC3851.03682396E-1014.33736438
GTCCTAG6050.014.259021
TCCGCAC8000.013.36847364
CGCACCT7900.013.10099366
CGCGCAT4801.8189894E-1112.9372340
GCAGCGT11500.012.9025541
CCGCACC9400.012.84547865
CTCGGTC5650.012.82274313
TATACTG25950.012.7653775
CCTCCTA8600.012.4385091
CTAATAC17800.012.4067983
CTAGACT7300.012.2899534
CGGTCAG5900.012.27940715
CTCCTAG6750.012.2690951
TAATACT27500.012.0458754
GTATATG24350.011.9037931
GGACCGT5552.0008883E-1111.81300456
Produced by FastQC (version 0.11.2)