FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614463
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20050.018.7589511
GCGTCAG13850.017.9385324
ACGCGCA7500.015.63958239
CGCGCAT8200.014.72521640
GACGCGC7650.014.43098638
TATACAG30400.014.30245
TAGGTAG12900.014.1774485
GTATTAA24700.013.5509971
ACCGCGC5100.013.5300148
GTATAGA25400.013.4492451
GTATATA38400.013.3891041
GCGCACT9050.013.3426611
CTAATAC25000.013.2505953
TAATACT40400.013.238984
TCCGCAC12550.013.19486764
GTATTAT37600.013.1233481
TATTATA38100.013.0419242
GCAGCGT19400.012.9842721
TATTAGG20200.012.8119162
CCGCACC13650.012.63702565
Produced by FastQC (version 0.11.2)