FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442037
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG3400.021.30836554
TCCTTGC30250.016.8803068
CGCGGGA4300.016.84847556
GTATCCT19750.016.0743391
GTATTAG18200.015.7368971
CGCGCAT4950.015.33300340
GCGCGGG4750.015.25230255
ACGCGCG4750.015.252130553
CCTCCTA8900.015.1212261
CCTTGCG13350.014.9891169
CTCCTAG7900.014.4144891
TCCGCAC8200.014.30440964
TCCTAGG5450.013.9299122
ATCCTTG37500.013.8927957
TAGACTG8950.013.881175
TAGGTAG8500.013.8040535
CGCACCT7850.013.6239166
TCTATAC21250.013.3165523
GGATCCT12500.013.2508471
CCTTCTA20350.013.2264291
Produced by FastQC (version 0.11.2)