FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7367051
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCG11700.025.94822136
GCGCACC12450.024.93928135
CCGGAGC12300.024.12116240
TCACGGG14000.022.17784519
GTATCCT31600.021.4026811
CACGGGC14200.021.37958120
ACGGGCC14400.021.08264221
CCTTGCG26050.019.7334989
CGGGCCG15800.018.77786622
TCCTTGC46600.018.7321078
CGCGCGG7200.018.68701454
GGCCGTC16100.018.64224624
CTCGGTC16400.018.30135313
CGGTCAG16450.018.24572815
AGCGCAC17600.018.03374534
CGCGGGA8100.017.88830256
GTATCCC15900.017.1446721
GGTCAGG17950.016.91309416
TACTCGG17900.016.76817711
GCACCGG19350.016.75936937
Produced by FastQC (version 0.11.2)