FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304293
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18500.016.4121231
TATTATA29100.014.93992
GCGTCAG13200.014.1160954
GTATAGA22500.013.9544481
TATTAGG20400.013.7001412
TCCTATA17050.013.558782
CTAATAC19800.013.2447323
GTATTAT33150.013.0100891
TAGGTAG11150.012.6883095
CGCGCAT6550.012.64092140
TATACTG29000.012.4935575
ATTATAC25650.012.3764243
GTATATA33700.012.2858491
CCTAATA19150.012.0719162
TATACAG24100.011.7406345
CTATACT20100.011.6736754
TAATACT28750.011.6420284
GTATAAA26450.011.6096231
ATTATAG26800.011.5878393
GACGCGC6850.011.58366638
Produced by FastQC (version 0.11.2)