FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577564
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA15750.016.211781
TATACAG18250.016.0714045
GTATTAG13900.015.6388671
CGCGCAT4250.015.42330540
TAGGTAG6050.014.2587815
GCGTCAG8450.013.4758124
TATACTG19950.013.31825
GTATATA22450.012.7567861
TATATAC23250.012.4667733
ACAGTGC10550.012.4274028
CGCGGGA3357.297476E-712.35785656
TATAGGA12600.012.32383252
TACACCG6450.012.3045545
TCCTTGC19150.012.2515018
CTAGACT7400.012.1238174
CTCCTAG6000.012.0766811
ATACAGG13750.012.0458186
TATTATA21800.012.0298692
GTATTAT23600.011.9889561
GCGCACT5501.6370905E-1111.91800711
Produced by FastQC (version 0.11.2)