FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471889
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCC851.4417207E-424.3524918
TGTACCG902.1209908E-423.0044525
CCTGCGT1352.6528323E-722.99957725
ATAGGGT953.062298E-421.7890748
CTCCCGA800.002893675821.5666736
ACCCTGC1605.7014404E-821.5621018
GTATTAG1304.9034406E-621.2348791
GCGGGGT1154.5959037E-520.99961513
CGGTCAG1154.5959037E-520.99961515
TGTACTA1004.3239378E-420.7040062
TATTAGG1356.7979254E-620.4484022
CTGGACC2201.891749E-1020.390314
CGCACCT850.00411708120.29374366
GTACCGT1751.4651596E-719.7181026
TATACTG2800.019.71815
GGTATAT1952.1414962E-819.4653051
CCCTGCC1952.1467713E-819.461189
GTCCTTA900.005723109519.1703781
TAGGGTG900.00573012419.1663159
CATGCGG1451.262423E-519.0341310
Produced by FastQC (version 0.11.2)