FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429431
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC5950.017.97468864
CCGCACC5950.017.97468865
GTATTAG15250.015.8375911
CGCACCT7050.015.17012666
CACCGCG3453.0559022E-1014.99991423
TATACAG29700.014.6388415
CTAATAC17000.013.8023373
GTATATA54050.013.7247271
GCTCGCG7200.013.41639561
ATTATAC30800.012.8836743
TATACTG24150.012.859325
ACTATGC18050.012.8067678
ACTGTGC8950.012.721348
CAGCGTG4953.274181E-1112.5455669
GTGTATA16150.012.3912871
GTGCGAC8650.012.36391129
GCGCAGC8150.012.27582456
ATACAGG19450.012.2411476
TTATACT25350.012.1144764
CAGCTCG8050.011.99975759
Produced by FastQC (version 0.11.2)