FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552157
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6300.025.743575
GTATTAG10150.024.1376651
TAGTCAG7100.022.3524889
ACATAGG6650.022.30777231
TTAGGTA8950.020.0485634
GTATAGA12000.019.266221
CTAATAC10800.019.1703683
CCTAGCG5800.019.03405830
TATTAGG10700.019.0266652
TAATACT22450.018.2908364
TATACAG16950.017.100985
GCGAGAC3451.8189894E-1216.99961940
CTAGGCA6700.016.99248910
CACCTAG6500.016.98423428
CCGAGGC5700.016.94698713
TCTATAC11200.016.9452343
TCGTGAG6350.016.84214232
CGATCAC6650.016.60113124
ACCTAGC6900.015.99964129
GTCGTGA6950.015.88453531
Produced by FastQC (version 0.11.2)