FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987349
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG4750.019.6137471
GCGTCAG7450.018.9893764
GTATCCT13500.017.3806321
TATTAGG9900.017.0779912
TAGGTAG5900.016.9604135
GTATTAG10500.016.7598951
TCCTTGC18700.016.6050598
CTAGACT4600.016.5024344
GGACCCG4200.016.42824445
CCCCTAG3351.9645086E-1015.4502991
CAGCGTC9800.015.1397342
GCAGCGT9950.014.5652071
CCTACAC6200.014.4698763
CCTTGCA15000.014.2601949
CGTCCGG4153.4197E-1013.300940532
CCTTGCG9600.013.2970569
CCCTCGG5200.013.26985558
CTAGACA11450.013.2595974
CCTCGGA7050.013.2129459
TATACAG13750.013.0494155
Produced by FastQC (version 0.11.2)