FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4673756
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18450.015.7097581
TCCGCAC7350.014.55063464
CCTACAC8400.013.9666063
GTATAGA23200.013.8318911
CCTATAC13000.013.8022923
TATTAGG20050.013.42338852
TAGGTAG10650.013.2847495
TCCTTGC26100.013.2194558
GCGCACT5800.013.08656111
CTATACT21650.013.0692854
CGCACCT8550.012.91193966
TATACCG14250.012.8345355
TCTATAC20250.012.77993
TAGACTG9500.012.7134535
CCGCACC8800.012.54512265
CCTTGCA19300.012.1561589
CGGTCGG3154.951886E-612.047313
CTATACA20950.012.0236054
CCTAGAC10050.012.016923
TAATACT33600.012.0155174
Produced by FastQC (version 0.11.2)