FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5673221
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA22500.015.795011
CTAATAC23700.015.5777413
GTATTAG19350.014.6217081
TATACCG14350.013.94615
CCTATAC18400.013.87659553
TATACAG25550.013.6397745
TAATACT35000.013.3089074
TCCTATA17200.013.2400112
CCTAATA23850.012.7311362
ACTATGC25550.012.6935488
GTATTAA26650.012.6880251
CTATACT21600.012.6197294
CGCACCT11700.012.38440266
TCCTAAG14800.012.3562582
TATACTG30450.012.3513915
GTATCCT18750.012.3293091
CTATGCA21850.012.3159049
CCGCACC12550.012.09540865
TAGGTAG10000.012.0766015
CGCGCGG4308.232746E-912.03467654
Produced by FastQC (version 0.11.2)