FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611264
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14300.016.4088521
GCGTCAG6900.016.0042844
TCCTTGC21350.013.57533558
TACGTCC6500.013.2727844
TAGGTAG7300.012.7638665
GTATCCT16550.012.7185361
TCCTATA12600.012.0506782
CCTTGCA18050.012.0417739
GTATAGA16850.011.8777281
GTATTAT22350.011.7338721
TATACAG18350.011.6599585
GTATATG17500.011.6337381
GTCCTAG6251.8189894E-1211.5943011
TATTATA22050.011.5811712
TATACCG9250.011.5654185
TACACTG14050.011.5441755
CTAATAC15900.011.5030793
CCCTAGA7200.011.502922
CCCCCTA5401.5097612E-1011.5022831
GTATATA22200.011.5022831
Produced by FastQC (version 0.11.2)