FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272103
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2802.5465852E-1017.24974340
CCGCGCG1452.7232824E-416.6556579
ACGCGCA3301.5643309E-1015.68158239
TGACGCG2654.1765816E-815.6227537
GTATTAG8500.015.4270321
TATACAG9950.014.2196245
CTAGGAC3403.981768E-914.2091093
TCTATAC9250.014.1761923
CCCCTAG2751.0758686E-613.8031341
CTAATAC8100.013.6327263
GTATACG7450.013.4325811
GACGCGC3355.0318704E-813.38785938
GCGTCAG4951.8189894E-1213.2454334
GTATAGT8150.013.1256811
CTATACA9750.013.0952824
GTATAGA10000.012.76789951
GTATATG11450.012.6578961
TCCTATA6850.012.5938232
CTTAAGG4953.274181E-1112.5483053
CGCATGA4701.8371793E-1012.47826242
Produced by FastQC (version 0.11.2)