FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529746
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC15750.017.9671383
CGCGCGG1855.4544107E-616.78344354
CTAATAC13500.016.1046923
GTATTAG14700.015.4932121
CTATACT15850.015.241254
GTATAGA17150.014.8895811
TACACCG8150.013.9735935
CCTACAC8100.013.6336013
TACACAG14300.013.5146195
CTATACA16350.013.0865684
TAGACAG13200.013.0721765
TATACTG20350.013.05804355
CCTATAC14850.013.0138923
CGCACCT5600.012.93723766
CCTAATA12550.012.92371752
GTCTTAG8650.012.76582051
GTATATG18000.012.652791
TATTAGG11750.012.6288542
CCTAGCG4651.546141E-1012.61246830
CTCTATA15350.012.589632
Produced by FastQC (version 0.11.2)